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Harlan Ellison, RIP
Last week Harlan Ellison died. He was one of my favorite writers for a long, long time.
I only have a couple of stories about interacting with him. But since so much of the responses to his death, both celebrations of his life and celebrations of his death have focused on Ellison as a person to interact with (see this MeFi thread for examples of both positive and negative reactions, or Cory Doctorow’s balanced account, or John Scalzi’s obituary), I’d rather talk about him as someone whose work I read, and who meant a lot to me.
Also, all of my books are in storage now because of the impending move (which is terrible, and I will write about this), so I can’t quickly reach for my Ellison paperbacks, hardcovers, CDs, and comic books. So his creative output looms especially large for me right now, just as he died.
If you don’t recognize the name, and haven’t clicked on the Wikipedia link above, know that Harlan Ellison was an important writer in many venues. He was best known for stories in science fiction, fantasy, and horror, but he also wrote mysteries, mainstream fiction, tv scripts, radio plays, essays on all sorts of things including tv, and a computer game. For some he’s most famous as the author of ev
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Abstract
Virus identification is a prerequisite not only for the early diagnosis of viral infectious diseases but also for the effective prevention of epidemics. Successful cultivation is the gold standard for identifying a virus, according to the Koch postulates. However, this requires screening for a permissive cell line, which is traditionally time-, reagent- and labor-intensive. Here, a simple and easy-to-operate microfluidic chip, formed by seeding a variety of cell lines and culturing them in parallel, is reported for use in virus cultivation and virus-permissive host-cell screening. The chip was tested by infection with two known viruses, enterovirus 71 (EV71) and influenza virus H1N1. Infection with EV71 and H1N1 caused significant cytopathic effects (CPE) in RD and MDCK cells, respectively, demonstrating that virus cultivation based on this microfluidic cell chip can be used as a substitute for the traditional plate-based culture method and reproduce the typical CPE caused by virus infection. Using this microfluidic cell chip method for virus cultivation could make it possible to identify an emerging virus in a high-throughput, automatic, and unprecedentedly fast way.
Keywords: Virus cultivation, Microfluidic cell chip, Permissive cell, Enterovirus 71 (EV71), Infl